Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

A549

biomaterial_provider

ATCC

biomaterial_type

immortalized cell line

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

controlid

wgEncodeEH003076

replicate

1,2

line

A549

cell organism

human

cell description

epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis

cell karyotype

cancer

cell lineage

endoderm

cell sex

M

antibody

Control

treatment

EtOH_0.02pct

treatment description

1 h with 0.02% Ethanol (Myers)

control

std

control description

Standard input signal for most experiments.

controlid

A549/EtOH_0.02pct/Input/std

labversion

Illumina_HiSeq_2000

labversion description

Illumina HiSeq 2000

replicate

1

Sequenced DNA Library

library_name

GSM1003541: Broad_ChipSeq_A549_Control_EtOH_0.02pct

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

188908119

Reads aligned (%)

96.3

Duplicates removed (%)

10.8

Number of peaks

3070 (qval < 1E-05)

hg19

Number of total reads

188908119

Reads aligned (%)

95.4

Duplicates removed (%)

12.9

Number of peaks

1928 (qval < 1E-05)