Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4K20me1

Cell type

Cell type Class
Blood
Cell type
Monocytes-CD14+
Tissue
monocytes
Lineage
mesoderm
Description
Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Attributes by original data submitter

Sample

source_name
Monocytes-CD14+_RO01746
biomaterial_provider
S. Heimfeld Laboratory, FHCRC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
rabbit polyclonal. Antibody Target: H4K20me1
antibody targetdescription
Histone H4 (mono-methyl K20). Is associated with active and accessible regions. In mammals, PR-Set7 specifically catalyzes H4K20 monomethylation. NOTE CONTRAST to H3K20me3 which is associated with heterochromatin and DNA repair.
antibody vendorname
abcam
antibody vendorid
ab9051
controlid
wgEncodeEH003130
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
H4K20me1
antibody antibodydescription
rabbit polyclonal. Antibody Target: H4K20me1
antibody targetdescription
Histone H4 (mono-methyl K20). Is associated with active and accessible regions. In mammals, PR-Set7 specifically catalyzes H4K20 monomethylation. NOTE CONTRAST to H3K20me3 which is associated with heterochromatin and DNA repair.
antibody vendorname
Abcam
antibody vendorid
ab9051
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
Monocytes-CD14+_RO01746/Input/std
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003539: Broad_ChipSeq_Monocytes-CD14+_RO01746_H4K20me1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
39698436
Reads aligned (%)
96.2
Duplicates removed (%)
31.0
Number of peaks
579 (qval < 1E-05)

hg19

Number of total reads
39698436
Reads aligned (%)
95.8
Duplicates removed (%)
31.6
Number of peaks
631 (qval < 1E-05)

Base call quality data from DBCLS SRA