Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

source_name

Monocytes-CD14+_RO01746

biomaterial_provider

S. Heimfeld Laboratory, FHCRC

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me1

antibody targetdescription

Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts.

antibody vendorname

abcam

antibody vendorid

ab8895

controlid

wgEncodeEH003130

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

F

antibody

H3K4me1

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me1

antibody targetdescription

Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts.

antibody vendorname

Abcam

antibody vendorid

ab8895

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

Monocytes-CD14+_RO01746/Input/std

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003535: Broad_ChipSeq_Monocytes-CD14+_RO01746_H3K4me1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

31981634

Reads aligned (%)

93.4

Duplicates removed (%)

22.2

Number of peaks

62169 (qval < 1E-05)

hg19

Number of total reads

31981634

Reads aligned (%)

93.2

Duplicates removed (%)

22.4

Number of peaks

61977 (qval < 1E-05)