Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3
antibody targetdescription
Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.
antibody vendorname
abcam
antibody vendorid
ab8898
controlid
wgEncodeEH000084
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
M
antibody
H3K9me3
antibody antibodydescription
rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3
antibody targetdescription
Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.
antibody vendorname
Abcam
antibody vendorid
ab8898
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
HepG2/None/Input/std
labversion
Illumina_HiSeq_2000
labversion description
Illumina HiSeq 2000
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003519: Broad_ChipSeq_HepG2_H3K9me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
142244403
Reads aligned (%)
94.7
Duplicates removed (%)
24.4
Number of peaks
5538 (qval < 1E-05)

hg19

Number of total reads
142244403
Reads aligned (%)
90.8
Duplicates removed (%)
31.9
Number of peaks
14710 (qval < 1E-05)

Base call quality data from DBCLS SRA