Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

HUVEC

biomaterial_provider

Lonza

donor_id

ENCDO000AAS,ENCDO551FXN

biomaterial_type

primary cell

cell_type

endothelial cell of umbilical vein

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3

antibody targetdescription

Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.

antibody vendorname

abcam

antibody vendorid

ab8898

controlid

wgEncodeEH000060

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

U

antibody

H3K9me3

antibody antibodydescription

rabbit polyclonal, ChIP grade. Antibody Target: H3K9me3

antibody targetdescription

Histone H3 (tri methyl K9). Is associated with repressive heterochromatic state (silenced chromatin). NOTE CONTRAST to H3K9me1 which is associated with active and accessible regions.

antibody vendorname

Abcam

antibody vendorid

ab8898

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

HUVEC/None/Input/std

labversion

Illumina_HiSeq_2000

labversion description

Illumina HiSeq 2000

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003517: Broad_ChipSeq_HUVEC_H3K9me3

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

80605012

Reads aligned (%)

83.7

Duplicates removed (%)

10.5

Number of peaks

3255 (qval < 1E-05)

hg19

Number of total reads

80605012

Reads aligned (%)

81.4

Duplicates removed (%)

15.1

Number of peaks

2254 (qval < 1E-05)