Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

source_name

Monocytes-CD14+_RO01746

biomaterial_provider

S. Heimfeld Laboratory, FHCRC

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me2

antibody targetdescription

Histone H3 (di methyl K4). Marks promoters and enhancers. Most CpG islands are marked by H3K4me2 in primary cells. May be associated also with poised promoters.

antibody vendorname

abcam

antibody vendorid

ab7766

controlid

wgEncodeEH003130

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

F

antibody

H3K4me2

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K4me2

antibody targetdescription

Histone H3 (di methyl K4). Marks promoters and enhancers. Most CpG islands are marked by H3K4me2 in primary cells. May be associated also with poised promoters.

antibody vendorname

Abcam

antibody vendorid

ab7766

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

Monocytes-CD14+_RO01746/None/Input/std

labversion

Illumina_HiSeq_2000

labversion description

Illumina HiSeq 2000

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

ERROR: cannot retrieve data from NCBI: too many requests

library_strategy

ERROR: cannot retrieve data from NCBI: too many requests

library_source

ERROR: cannot retrieve data from NCBI: too many requests

library_selection

ERROR: cannot retrieve data from NCBI: too many requests

library_construction_protocol

ERROR: cannot retrieve data from NCBI: too many requests

Sequencing Platform

instrument_model

ERROR: cannot retrieve data from NCBI: too many requests

cycle_sequence

ERROR: cannot retrieve data from NCBI: too many requests

cycle_count

ERROR: cannot retrieve data from NCBI: too many requests

flow_sequence

ERROR: cannot retrieve data from NCBI: too many requests

flow_count

ERROR: cannot retrieve data from NCBI: too many requests

key_sequence

ERROR: cannot retrieve data from NCBI: too many requests

hg38

Number of total reads

58149271

Reads aligned (%)

96.5

Duplicates removed (%)

52.4

Number of peaks

53645 (qval < 1E-05)

hg19

Number of total reads

58149271

Reads aligned (%)

96.3

Duplicates removed (%)

52.7

Number of peaks

53496 (qval < 1E-05)