Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Bone
Cell type
Osteobl
Tissue
bone
Lineage
mesoderm
Description
osteoblasts (NHOst)

Attributes by original data submitter

Sample

source_name
Osteobl
biomaterial_provider
Lonza
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit Polyclonal Antigen Affinity Purified, Unconjugated, Liquid
antibody targetdescription
Histone acetyltransferase with sequence similarity to CBP (CREB-binding protein). Regulates transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation.
antibody vendorname
Bethyl Laboratories
antibody vendorid
A300-358A
controlid
wgEncodeEH001056
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
U
antibody
P300_KAT3B
antibody antibodydescription
Rabbit Polyclonal Antigen Affinity Purified, Unconjugated, Liquid
antibody targetdescription
Histone acetyltransferase with sequence similarity to CBP (CREB-binding protein). Regulates transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation.
antibody vendorname
Bethyl
antibody vendorid
A300-358A
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
Osteobl/None/Input/std
labversion
Illumina_HiSeq_2000
labversion description
Illumina HiSeq 2000
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003514: Broad_ChipSeq_Osteobl_P300_KAT3B
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
62557246
Reads aligned (%)
87.6
Duplicates removed (%)
50.2
Number of peaks
5092 (qval < 1E-05)

hg38

Number of total reads
62557246
Reads aligned (%)
88.2
Duplicates removed (%)
48.8
Number of peaks
5194 (qval < 1E-05)

Base call quality data from DBCLS SRA