Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Bone
Cell type
Osteobl
Tissue
bone
Lineage
mesoderm
Description
osteoblasts (NHOst)

Attributes by original data submitter

Sample

source_name
Osteobl
biomaterial_provider
Lonza
donor_id
ENCDO834SRG,ENCDO271AAA
biomaterial_type
primary cell
cell_type
osteoblast
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
1)abcam; 2)Millipore
antibody vendorid
ab8580; 07-473
controlid
wgEncodeEH001056
replicate
1,3,2
softwareversion
ScriptureVPaperR3
cell sex
U
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
Osteobl/None/Input/std
labversion
Illumina_GA2e
labversion description
Illumina Genome Analyzer IIe
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003506: Broad_ChipSeq_Osteobl_H3K4me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
89121946
Reads aligned (%)
83.5
Duplicates removed (%)
17.1
Number of peaks
44621 (qval < 1E-05)

hg19

Number of total reads
89121946
Reads aligned (%)
83.0
Duplicates removed (%)
18.4
Number of peaks
45245 (qval < 1E-05)

Base call quality data from DBCLS SRA