Antigen

Antigen Class

Histone

Antigen

H2A.Z

Cell type

Cell type Class

Blood

Cell type

CD20+

Tissue

blood

Lineage

mesoderm

Description

B cells from donors RO01778 and RO01794, newly promoted to tier 2: not in 2011 analysis

Sample

source_name

CD20+

biomaterial_provider

Dr. Heimfeld Lab at FHCRC

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

Rabbit polyclonal antibody raised against a peptide corresponding to the C-terminus of H2AZ. Antibody Target: H2AZ

antibody targetdescription

H2A.Z is a sequence variant of Histone H2A.

antibody vendorname

Millipore

antibody vendorid

07-594

controlid

wgEncodeEH003132

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

F

antibody

H2A.Z

antibody antibodydescription

Rabbit polyclonal antibody raised against a peptide corresponding to the C-terminus of H2AZ. Antibody Target: H2AZ

antibody targetdescription

H2A.Z is a sequence variant of Histone H2A.

antibody vendorname

Upstate (Millipore)

antibody vendorid

07-594

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

CD20+/None/Input/std

labversion

Illumina_HiSeq_2000

labversion description

Illumina HiSeq 2000

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003476: Broad_ChipSeq_CD20+_H2A.Z

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

49368912

Reads aligned (%)

84.0

Duplicates removed (%)

30.2

Number of peaks

32032 (qval < 1E-05)

hg19

Number of total reads

49368912

Reads aligned (%)

83.5

Duplicates removed (%)

31.2

Number of peaks

32020 (qval < 1E-05)