Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Blood
Cell type
CD20+
Tissue
blood
Lineage
mesoderm
Description
B cells from donors RO01778 and RO01794, newly promoted to tier 2: not in 2011 analysis

Attributes by original data submitter

Sample

source_name
CD20+
biomaterial_provider
Dr. Heimfeld Lab at FHCRC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me2
antibody targetdescription
Histone H3 (di methyl K4). Marks promoters and enhancers. Most CpG islands are marked by H3K4me2 in primary cells. May be associated also with poised promoters.
antibody vendorname
abcam
antibody vendorid
ab7766
controlid
wgEncodeEH003132
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
H3K4me2
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me2
antibody targetdescription
Histone H3 (di methyl K4). Marks promoters and enhancers. Most CpG islands are marked by H3K4me2 in primary cells. May be associated also with poised promoters.
antibody vendorname
Abcam
antibody vendorid
ab7766
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
CD20+/None/Input/std
labversion
Illumina_HiSeq_2000
labversion description
Illumina HiSeq 2000
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003471: Broad_ChipSeq_CD20+_H3K4me2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
99740633
Reads aligned (%)
92.5
Duplicates removed (%)
42.0
Number of peaks
57457 (qval < 1E-05)

hg19

Number of total reads
99740633
Reads aligned (%)
92.1
Duplicates removed (%)
42.7
Number of peaks
57229 (qval < 1E-05)

Base call quality data from DBCLS SRA