Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Blood
Cell type
CD20+
Tissue
blood
Lineage
mesoderm
Description
B cells from donors RO01778 and RO01794, newly promoted to tier 2: not in 2011 analysis

Attributes by original data submitter

Sample

source_name
CD20+_RO01794
biomaterial_provider
Rajinder Kaul, UW
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2. Antibody Target: EZH2
antibody targetdescription
EZH2 (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.
antibody vendorname
Active Motif
antibody vendorid
39875
controlid
wgEncodeEH003132
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
F
antibody
EZH2_(39875)
antibody antibodydescription
This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2. Antibody Target: EZH2
antibody targetdescription
EZH2 (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.
antibody vendorname
Active Motif
antibody vendorid
39875
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
CD20+_RO01794/None/Input/std
labversion
Illumina_HiSeq_2000
labversion description
Illumina HiSeq 2000
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003470: Broad_ChipSeq_CD20+_RO01794_EZH2_(39875)
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
68261445
Reads aligned (%)
44.3
Duplicates removed (%)
48.6
Number of peaks
1534 (qval < 1E-05)

hg38

Number of total reads
68261445
Reads aligned (%)
46.6
Duplicates removed (%)
46.5
Number of peaks
1551 (qval < 1E-05)

Base call quality data from DBCLS SRA