Antigen

Antigen Class

TFs and others

Antigen

EZH2

Cell type

Cell type Class

Blood

Cell type

CD20+

Tissue

blood

Lineage

mesoderm

Description

B cells from donors RO01778 and RO01794, newly promoted to tier 2: not in 2011 analysis

Sample

source_name

CD20+_RO01794

biomaterial_provider

Rajinder Kaul, UW

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2. Antibody Target: EZH2

antibody targetdescription

EZH2 (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

antibody vendorname

Active Motif

antibody vendorid

39875

controlid

wgEncodeEH003132

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

F

antibody

EZH2_(39875)

antibody antibodydescription

This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2. Antibody Target: EZH2

antibody targetdescription

EZH2 (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

antibody vendorname

Active Motif

antibody vendorid

39875

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

CD20+_RO01794/None/Input/std

labversion

Illumina_HiSeq_2000

labversion description

Illumina HiSeq 2000

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003470: Broad_ChipSeq_CD20+_RO01794_EZH2_(39875)

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

68261445

Reads aligned (%)

87.1

Duplicates removed (%)

46.5

Number of peaks

1551 (qval < 1E-05)

hg19

Number of total reads

68261445

Reads aligned (%)

86.2

Duplicates removed (%)

48.6

Number of peaks

1534 (qval < 1E-05)