Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

CD20+

Tissue

blood

Lineage

mesoderm

Description

B cells from donors RO01778 and RO01794, newly promoted to tier 2: not in 2011 analysis

Sample

source_name

CD20+_RO01794

biomaterial_provider

Rajinder Kaul, UW

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K27ac

antibody targetdescription

Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.

antibody vendorname

abcam

antibody vendorid

ab4729

controlid

wgEncodeEH003132

replicate

1,2

softwareversion

ScriptureVPaperR3

cell sex

F

antibody

H3K27ac

antibody antibodydescription

rabbit polyclonal. Antibody Target: H3K27ac

antibody targetdescription

Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.

antibody vendorname

Abcam

antibody vendorid

ab4729

treatment

None

treatment description

No special treatment or protocol applies

control

std

control description

Standard input signal for most experiments.

controlid

CD20+_RO01794/None/Input/std

labversion

Illumina_HiSeq_2000

labversion description

Illumina HiSeq 2000

softwareversion

ScriptureVPaperR3

Sequenced DNA Library

library_name

GSM1003459: Broad_ChipSeq_CD20+_RO01794_H3K27ac

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

121466498

Reads aligned (%)

87.4

Duplicates removed (%)

23.6

Number of peaks

34484 (qval < 1E-05)

hg19

Number of total reads

121466498

Reads aligned (%)

86.8

Duplicates removed (%)

24.6

Number of peaks

34223 (qval < 1E-05)