Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KDM5B

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Polyclonal Antigen Affinity Purified, Unconjugated, Liquid. Antibody Target: PLU1
antibody targetdescription
JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.
antibody vendorname
YangShi lab
antibody vendorid
PLU1
controlid
wgEncodeEH000088
replicate
1
softwareversion
ScriptureVPaperR3
cell sex
M
antibody
PLU1
antibody antibodydescription
Polyclonal Antigen Affinity Purified, Unconjugated, Liquid. Antibody Target: PLU1
antibody targetdescription
JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer. Alternative names for JARID1B include retinoblastoma-binding protein 2 homolog 1, RBP2-H1, cancer/testis antigen 31, CT31, PLU-1, PUT1, and KDM5B.
antibody vendorname
YangShi lab
antibody vendorid
PLU1
treatment
None
treatment description
No special treatment or protocol applies
control
std
control description
Standard input signal for most experiments.
controlid
H1-hESC/None/Input/std
labversion
Illumina_GA2x
labversion description
Illumina Genome Analyzer IIx
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003457: Broad_ChipSeq_H1-hESC_PLU1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
54743858
Reads aligned (%)
43.7
Duplicates removed (%)
42.6
Number of peaks
1260 (qval < 1E-05)

hg38

Number of total reads
54743858
Reads aligned (%)
45.2
Duplicates removed (%)
41.1
Number of peaks
1214 (qval < 1E-05)

Base call quality data from DBCLS SRA