Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
biomaterial_provider
ATCC
biomaterial_type
immortalized cell line
line
A549
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me1
antibody targetdescription
Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts.
antibody vendorname
abcam
antibody vendorid
ab8895
controlid
wgEncodeEH003076
replicate
1,2
softwareversion
ScriptureVPaperR3
cell sex
M
antibody
H3K4me1
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me1
antibody targetdescription
Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts.
antibody vendorname
Abcam
antibody vendorid
ab8895
treatment
EtOH_0.02pct
treatment description
1 h with 0.02% Ethanol (Myers)
control
std
control description
Standard input signal for most experiments.
controlid
A549/EtOH_0.02pct/Input/std
labversion
Illumina_HiSeq_2000
labversion description
Illumina HiSeq 2000
softwareversion
ScriptureVPaperR3

Sequenced DNA Library

library_name
GSM1003453: Broad_ChipSeq_A549_H3K4me1_EtOH_0.02pct
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
103695721
Reads aligned (%)
95.3
Duplicates removed (%)
11.2
Number of peaks
66907 (qval < 1E-05)

hg19

Number of total reads
103695721
Reads aligned (%)
94.9
Duplicates removed (%)
11.9
Number of peaks
66603 (qval < 1E-05)

Base call quality data from DBCLS SRA