Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CHD1

Cell type

Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
IMR90
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
IMR90
cell organism
human
cell description
fetal lung fibroblasts, newly promoted to tier 2: not in 2011 analysis
cell karyotype
normal
cell lineage
endoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
CHD1_(NB100-60411)
antibody antibodydescription
Rabbit polyclonal, The epitope recognized by this antibody maps to a region between residue 1660 and 1710 of human chromodomain helicase DNA binding protein 1 using the numbering given in entry NP_001261.2 (GeneID 1105). Antibody Target: CHD1
antibody targetdescription
ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells
antibody vendorname
Novus Biologicals
antibody vendorid
NB100-60411
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH002808

Sequenced DNA Library

library_name
GSM1003623: Stanford_ChipSeq_IMR90_CHD1_(NB100-60411)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
51307688
Reads aligned (%)
86.3
Duplicates removed (%)
10.3
Number of peaks
2511 (qval < 1E-05)

hg38

Number of total reads
51307688
Reads aligned (%)
88.6
Duplicates removed (%)
8.7
Number of peaks
5987 (qval < 1E-05)

Base call quality data from DBCLS SRA