Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ELK1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
ELK1_(1277-1)
antibody antibodydescription
Rabbit polyclonal, a synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members. Antibody Target: ELK1
antibody targetdescription
Elk-1 is a transcription factor that is part of the ets oncogene superfamily. Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors. Activation by several mitogen- activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli.
antibody vendorname
Epitomics
antibody vendorid
1277-1
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
labversion
PeakSeq1.0

Sequenced DNA Library

library_name
GSM1003620: Stanford_ChipSeq_K562_ELK1_(1277-1)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
48194999
Reads aligned (%)
87.2
Duplicates removed (%)
5.7
Number of peaks
3209 (qval < 1E-05)

hg38

Number of total reads
48194999
Reads aligned (%)
89.0
Duplicates removed (%)
4.6
Number of peaks
3047 (qval < 1E-05)

Base call quality data from DBCLS SRA