Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RFX5

Cell type

Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
IMR90
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
IMR90
cell organism
human
cell description
fetal lung fibroblasts, newly promoted to tier 2: not in 2011 analysis
cell karyotype
normal
cell lineage
endoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
RFX5_(200-401-194)
antibody antibodydescription
Rabbit polyclonal against RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin. Antibody Target: RFX5
antibody targetdescription
Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding betweenRFX and NF-Y. RFX binds the X1 box of MHC-II promoters
antibody vendorname
Rockland Immunochemicals
antibody vendorid
200-401-194
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH002808

Sequenced DNA Library

library_name
GSM1003615: Stanford_ChipSeq_IMR90_RFX5_(200-401-194)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
61119489
Reads aligned (%)
74.0
Duplicates removed (%)
18.6
Number of peaks
3849 (qval < 1E-05)

hg38

Number of total reads
61119489
Reads aligned (%)
75.9
Duplicates removed (%)
17.1
Number of peaks
3721 (qval < 1E-05)

Base call quality data from DBCLS SRA