Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RCOR1

Cell type

Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
IMR90
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
IMR90
cell organism
human
cell description
fetal lung fibroblasts, newly promoted to tier 2: not in 2011 analysis
cell karyotype
normal
cell lineage
endoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
COREST_(sc-30189)
antibody antibodydescription
Rabbit polyclonal, epitope corresponding to amino acids 246-310 mapping within an internal region of CoREST of human origin recommended for detection of CoREST of mouse, rat and human origin. Antibody Target: COREST (sc30189)
antibody targetdescription
Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-30189
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH002808

Sequenced DNA Library

library_name
GSM1003612: Stanford_ChipSeq_IMR90_COREST_(sc-30189)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
62296651
Reads aligned (%)
80.0
Duplicates removed (%)
14.9
Number of peaks
14735 (qval < 1E-05)

hg38

Number of total reads
62296651
Reads aligned (%)
82.2
Duplicates removed (%)
13.3
Number of peaks
14705 (qval < 1E-05)

Base call quality data from DBCLS SRA