Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Spleen
Cell type
Spleen
MeSH Description
An encapsulated lymphatic organ through which venous blood filters.

Attributes by original data submitter

Sample

source_name
Spleen
biomaterial_provider
1) LICR lab; 2)Stam Lab; 3)CSHL lab
lab
LICR-m
lab description
Ren - Ludwig Institue for Cancer Research
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
biomaterial_type
tissue
donor_id
ENCDO956IXV
cell organism
mouse
tissue_type
Spleen
Sex
male
antibody
H3K27me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27me3
antibody targetdescription
Histone H3 (tri-methyl K27). Marks promoters that are silenced by Polycomb proteins in a given lineage; large domains are found at inactive developmental loci.
antibody vendorname
Millipore
antibody vendorid
07-449
age
adult-8wks
age description
Adult 8 weeks
age
adult-8wks
age description
Adult 8 weeks
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEM001459
labversion
Pooled & filtered
softwareversion
MACS
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM1000146: LICR_ChipSeq_Spleen_H3K27me3_adult-8wks
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
84364997
Reads aligned (%)
87.9
Duplicates removed (%)
37.5
Number of peaks
825 (qval < 1E-05)

mm9

Number of total reads
84364997
Reads aligned (%)
87.7
Duplicates removed (%)
37.4
Number of peaks
975 (qval < 1E-05)

Base call quality data from DBCLS SRA