Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
LICR-m
lab description
Ren - Ludwig Institue for Cancer Research
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
line
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
H3K27ac
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription
Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname
Abcam
antibody vendorid
ab4729
age
immortalized
biomaterial_type
immortalized cell line
age
immortalized
biomaterial_type
immortalized cell line
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEM003164
labversion
Pooled & filtered
softwareversion
MACS
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM1000117: LICR_ChipSeq_CH12_H3K27ac_immortalized
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
41642165
Reads aligned (%)
87.9
Duplicates removed (%)
27.5
Number of peaks
29212 (qval < 1E-05)

mm9

Number of total reads
41642165
Reads aligned (%)
87.8
Duplicates removed (%)
27.6
Number of peaks
29258 (qval < 1E-05)

Base call quality data from DBCLS SRA