Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Embryo
Cell type
Embryonic limb
NA
NA

Attributes by original data submitter

Sample

source_name
Limb
biomaterial_provider
1)LICR lab; 2)CSHL lab
lab
LICR-m
lab description
Ren - Ludwig Institue for Cancer Research
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
Limb
cell organism
mouse
cell description
Limb
cell sex
U
antibody
H3K4me1
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me1
antibody targetdescription
Histone H3 (mono methyl K4). Is associated with enhancers, and downstream of transcription starts.
antibody vendorname
Abcam
antibody vendorid
ab8895
age
E14.5
age description
Embryonic day 14.5
age
E14.5
age description
Embryonic day 14.5
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEM002482
labversion
Pooled & filtered
softwareversion
MACS
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM1000085: LICR_ChipSeq_Limb_H3K4me1_E14.5
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
29250186
Reads aligned (%)
63.6
Duplicates removed (%)
6.8
Number of peaks
11526 (qval < 1E-05)

mm9

Number of total reads
29250186
Reads aligned (%)
63.6
Duplicates removed (%)
6.9
Number of peaks
11492 (qval < 1E-05)

Base call quality data from DBCLS SRA