Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Spleen
Cell type
Spleen
MeSH Description
An encapsulated lymphatic organ through which venous blood filters.

Attributes by original data submitter

Sample

source_name
Spleen
biomaterial_provider
1) LICR lab; 2)Stam Lab; 3)CSHL lab
lab
LICR-m
lab description
Ren - Ludwig Institue for Cancer Research
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
Spleen
cell organism
mouse
cell description
Spleen
cell sex
M
antibody
H3K36me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K36me3
antibody targetdescription
Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname
Abcam
antibody vendorid
ab9050
age
adult-8wks
age description
Adult 8 weeks
age
adult-8wks
age description
Adult 8 weeks
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEM001459
labversion
Pooled & filtered
softwareversion
MACS
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM1000070: LICR_ChipSeq_Spleen_H3K36me3_adult-8wks
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
104747550
Reads aligned (%)
90.8
Duplicates removed (%)
21.3
Number of peaks
1221 (qval < 1E-05)

mm9

Number of total reads
104747550
Reads aligned (%)
90.7
Duplicates removed (%)
21.3
Number of peaks
1152 (qval < 1E-05)

Base call quality data from DBCLS SRA