Antigen

Antigen Class

TFs and others

Antigen

GUCY1B3

Cell type

Cell type Class

Neural

Cell type

U-87 MG

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

U-87 MG glioma cancer cells overexpressing sGCβ1, sGCβ1 ChIP

originating cell line

U-87 MG

tissue derivation

malignant glioma

cell type

glioma cancer cell line overexpressing sGC-beta1

chip antibody

anti-sGC-beta1 (Sigma G4405, lot 021M4843)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and chromatins were isolated with anti-sGCβ1 antibody (Sigma G4405). The ChIP DNA was processed into a standard Illumina ChIP-Seq library.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

29167193

Reads aligned (%)

97.2

Duplicates removed (%)

25.6

Number of peaks

1791 (qval < 1E-05)

hg19

Number of total reads

29167193

Reads aligned (%)

96.2

Duplicates removed (%)

27.7

Number of peaks

1266 (qval < 1E-05)