GSM2202475: Input for S2 cells treated with GFP RNAi; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage
Attributes by original data submitter
Sample
source_name
S2 cells
treatment
GFP dsRNA treated S2 cells
antibody
none
cell type
S2 cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Kc cells were washed with PBS and croslinked with formaldehyde (1%). Formaldehyde was quenched with glycine. Cells were washed twice with ice-cold PBS. Nuclei were exrtacted with IP buffer . Nuclei were sheared and incubated with antibody overnight. immunocomplexes were collected by agarose A/G beads. DNA was purified with Chelex-100 beads. Libraries were prepared according to Illumina's instructions