Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gcn5

Cell type

Cell type Class
Cell line
Cell type
Kc167
Source
e/se
Developmental Stage
dorsal closure stage

Attributes by original data submitter

Sample

source_name
Kc cells
treatment
GFP dsRNA treated Kc cells
antibody
Gcn5 rabbit polyclonal antibody (5 ug/IP)
cell type
Kc cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Kc cells were washed with PBS and croslinked with formaldehyde (1%). Formaldehyde was quenched with glycine. Cells were washed twice with ice-cold PBS. Nuclei were exrtacted with IP buffer . Nuclei were sheared and incubated with antibody overnight. immunocomplexes were collected by agarose A/G beads. DNA was purified with Chelex-100 beads. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
19949999
Reads aligned (%)
35.5
Duplicates removed (%)
58.4
Number of peaks
10363 (qval < 1E-05)

dm6

Number of total reads
19949999
Reads aligned (%)
34.4
Duplicates removed (%)
59.5
Number of peaks
10075 (qval < 1E-05)

Base call quality data from DBCLS SRA