Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ep300

Cell type

Cell type Class
Blood
Cell type
Th1 Cells
MeSH Description
A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Attributes by original data submitter

Sample

source_name
primary CD4+ T cells from spleen and lymph nodes
strain
C57BL/6J
genotype/variation
Tbet<tm1> (TbetKO)
cell type
in vitro polarized T helper1 cells for 7 days
passages
FACS sorted naive CD4+T cells cultured in vitro for 7 days under Th1 condition and restimulated with plate-bound CD3/28 +IL12 for 2 hrs
chip antibody
anti-p300

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
40049789
Reads aligned (%)
97.7
Duplicates removed (%)
29.3
Number of peaks
53292 (qval < 1E-05)

mm9

Number of total reads
40049789
Reads aligned (%)
97.6
Duplicates removed (%)
29.4
Number of peaks
53250 (qval < 1E-05)

Base call quality data from DBCLS SRA