GSM2197641: Pc ChIP-seq in wild-type cells replicate2 [part 3]; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Pc
Cell type
Cell type Class
Cell line
Cell type
Cultured cell lines
NA
NA
Attributes by original data submitter
Sample
source_name
Cultured cells, WT, Pc ChIP
cell line
Ras3
mutation
none
chip antibody
Pc from Poux et al., 2001
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Live cells were crosslinked with 1.8% formaldehyde for 10 min at 25C and soluble chromatin prepared as described in Schwartz et al., 2006. ChIP was done as described in Schwartz et al., 2006. Libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos for Illumina (New England BioLabs) following the manufacturer instructions.