Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Embryo

Cell type

Embryonic limb

NA

NA

Sample

age

11.5 day

dev stage

embryonic

donor ID

ENCDO956IXV

tissue

limb

sex

pooled male and female

health state

healthy

sample type

tissue

lab

John Stamatoyannopoulos, UW

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

document: https://www.encodeproject.org/documents/926174f5-d14c-4e77-bc52-5517b56daac0/@@download/attachment/Culturedcells_SOP_nuclei_DNase_crosslink_RNA_V1.pdf See ENCODE portal

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

220611348

Reads aligned (%)

73.2

Duplicates removed (%)

24.3

Number of peaks

63621 (qval < 1E-05)

mm9

Number of total reads

220611348

Reads aligned (%)

73.1

Duplicates removed (%)

24.5

Number of peaks

63695 (qval < 1E-05)