Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
INO80

Cell type

Cell type Class
Epidermis
Cell type
Melanocytes
MeSH Description
Mammalian pigment cells that produce MELANINS, pigments found mainly in the EPIDERMIS, but also in the eyes and the hair, by a process called melanogenesis. Coloration can be altered by the number of melanocytes or the amount of pigment produced and stored in the organelles called MELANOSOMES. The large non-mammalian melanin-containing cells are called MELANOPHORES.

Attributes by original data submitter

Sample

source_name
Melanocyte
chip antibody
Ino80
chip antibody vendor
proteintech

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed, harvested, and lysed, then submitted to sonication. The sonicated DNA was incubated with specific beads-conjugated antibody overnight. Then the beads were washed, and DNA was eluted, and quantified. The sequencing library was generated by use of Nextera XT DNA preparation Kit Nextera XT DNA preparation Kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
30972390
Reads aligned (%)
116.8
Duplicates removed (%)
11.3
Number of peaks
408 (qval < 1E-05)

hg38

Number of total reads
30972390
Reads aligned (%)
119.3
Duplicates removed (%)
10.8
Number of peaks
608 (qval < 1E-05)

Base call quality data from DBCLS SRA