Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MED1

Cell type

Cell type Class
Epidermis
Cell type
A-375
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
human A375
chip antibody
Med1
chip antibody vendor
Bethyl

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed, harvested, and lysed, then submitted to sonication. The sonicated DNA was incubated with specific beads-conjugated antibody overnight. Then the beads were washed, and DNA was eluted, and quantified. The sequencing library was generated by use of Nextera XT DNA preparation Kit Nextera XT DNA preparation Kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
32315811
Reads aligned (%)
51.7
Duplicates removed (%)
56.5
Number of peaks
341 (qval < 1E-05)

hg38

Number of total reads
32315811
Reads aligned (%)
53.6
Duplicates removed (%)
55.4
Number of peaks
553 (qval < 1E-05)

Base call quality data from DBCLS SRA