GSM2190583: A375 shNT Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Epidermis
Cell type
A-375
Primary Tissue
Skin
Tissue Diagnosis
Melanoma
Attributes by original data submitter
Sample
source_name
human A375
cell line
human A375
cell type
melanoma cell line
infected with
lenti-viral based shNT
time point
48 hours after infection
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed, harvested, and lysed, then submitted to sonication. The sonicated DNA was incubated with specific beads-conjugated antibody overnight. Then the beads were washed, and DNA was eluted, and quantified. The sequencing library was generated by use of Nextera XT DNA preparation Kit Nextera XT DNA preparation Kit