Antigen

Antigen Class

TFs and others

Antigen

MED1

Cell type

Cell type Class

Epidermis

Cell type

A-375

Primary Tissue

Skin

Tissue Diagnosis

Melanoma

Sample

source_name

human A375

chip antibody

Med1

chip antibody vendor

Bethyl

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed, harvested, and lysed, then submitted to sonication. The sonicated DNA was incubated with specific beads-conjugated antibody overnight. Then the beads were washed, and DNA was eluted, and quantified. The sequencing library was generated by use of Nextera XT DNA preparation Kit Nextera XT DNA preparation Kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

30727945

Reads aligned (%)

61.4

Duplicates removed (%)

55.6

Number of peaks

535 (qval < 1E-05)

hg19

Number of total reads

30727945

Reads aligned (%)

60.6

Duplicates removed (%)

56.7

Number of peaks

338 (qval < 1E-05)