Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP Cell Line
cell line
LNCaP
cell cycle arrest treatment
2μg/mL aphidicolin for 15 hours, followed by washout and treatment with 50ng/mL nocodozole for 9 hours +3 hour 10nm DHT
dht treatment
+
chip antibody
AR N-20 (SC-816)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as previously described by Schmidt et al 2009 Illumina library prep kit was utilyzed according to manufacturer's instructions

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
28686071
Reads aligned (%)
75.7
Duplicates removed (%)
19.7
Number of peaks
38610 (qval < 1E-05)

hg19

Number of total reads
28686071
Reads aligned (%)
75.3
Duplicates removed (%)
20.5
Number of peaks
38508 (qval < 1E-05)

Base call quality data from DBCLS SRA