Antigen

Antigen Class

TFs and others

Antigen

Gata1

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4

biomaterial_provider

Mitch Weiss lab

lab

PSU-m

lab description

Hardison - Penn State University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

G1E-ER4

cell organism

mouse

cell description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

cell sex

M

antibody

GATA1_(SC-265)

antibody antibodydescription

rat monoclonal antibody raised against mouse recombinant GATA1; recommended for detection of GATA1 of mouse, rat and human origin. Antibody Target: GATA1

antibody targetdescription

GATA1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes.

antibody vendorname

Santa Cruz Biotech

antibody vendorid

sc-265

treatment

None

treatment description

No special treatment or protocol applies

labexpid

Timecourse

age

E0

age description

Embryonic day 0 (stem cell)

control

Input

control description

Control signal which may be subtracted from experimental raw signal before peaks are called.

controlid

wgEncodeEM003202

labexpid

Timecourse

replicate

1

strain

129

strain description

Strain 129, has widely available embryonic stem cells

Sequenced DNA Library

library_name

GSM995445: PSU_ChipSeq_G1E-ER4_GATA1_(SC-265)_Timecourse

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

38492124

Reads aligned (%)

93.9

Duplicates removed (%)

8.3

Number of peaks

5836 (qval < 1E-05)

mm9

Number of total reads

38492124

Reads aligned (%)

93.8

Duplicates removed (%)

8.3

Number of peaks

5841 (qval < 1E-05)