Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gata1

Cell type

Cell type Class
Blood
Cell type
G1E-ER4+E2
Tissue
Blood
Lineage
cellLine
Description
Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Attributes by original data submitter

Sample

source_name
G1E-ER4
biomaterial_provider
Mitch Weiss lab
lab
PSU-m
lab description
Hardison - Penn State University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
G1E-ER4
cell organism
mouse
cell description
Gata1 restored erythroid cells, differentiation induced by estradiol (E2)
cell sex
M
antibody
GATA1_(SC-265)
antibody antibodydescription
rat monoclonal antibody raised against mouse recombinant GATA1; recommended for detection of GATA1 of mouse, rat and human origin. Antibody Target: GATA1
antibody targetdescription
GATA1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-265
treatment
diffProtD_7hr
treatment description
7 hours differentiation of G1E-ER4 cells with 10 nM beta-estradiol (Hardison)
labexpid
Timecourse
age
E0
age description
Embryonic day 0 (stem cell)
control
Input
control description
Control signal which may be subtracted from experimental raw signal before peaks are called.
controlid
wgEncodeEM003207
labexpid
Timecourse
replicate
1
strain
129
strain description
Strain 129, has widely available embryonic stem cells

Sequenced DNA Library

library_name
GSM995442: PSU_ChipSeq_G1E-ER4_GATA1_(SC-265)_diffProtD_7hr_Timecourse
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
34012672
Reads aligned (%)
96.8
Duplicates removed (%)
10.9
Number of peaks
9206 (qval < 1E-05)

mm9

Number of total reads
34012672
Reads aligned (%)
96.6
Duplicates removed (%)
10.9
Number of peaks
9262 (qval < 1E-05)

Base call quality data from DBCLS SRA