Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Digestive tract
Cell type
Colon
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse
tissue
colon
chip antibody
input
sample type
DNA control

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to instructions of NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #E6240S/L)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
9248507
Reads aligned (%)
94.7
Duplicates removed (%)
20.9
Number of peaks
8139 (qval < 1E-05)

mm9

Number of total reads
9248507
Reads aligned (%)
94.3
Duplicates removed (%)
21.2
Number of peaks
8085 (qval < 1E-05)

Base call quality data from DBCLS SRA