Antigen

Antigen Class

TFs and others

Antigen

Atoh1

Cell type

Cell type Class

Digestive tract

Cell type

Small intestine

NA

NA

Sample

source_name

Mouse

tissue

distal small intestine [Ileum]

chip antibody

GFP

sample type

Atoh1 Binding sites

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to instructions of NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB #E6240S/L)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

8505411

Reads aligned (%)

63.9

Duplicates removed (%)

81.2

Number of peaks

804 (qval < 1E-05)

mm9

Number of total reads

8505411

Reads aligned (%)

63.0

Duplicates removed (%)

81.9

Number of peaks

835 (qval < 1E-05)