Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

Preimplantation embryo

NA

NA

Sample

source_name

Preimplantation embryo

strain

C57BL/6N x PWK

developmental stage

Preimplantation embryo

chip antibody

H3K27Me3

chip antibody vendor

ActiveMotif

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. with slightly modification (Peng, Xu, et al. "TELP, a sensitive and versatile library construction method for next-generation sequencing." Nucleic acids research (2014): gku827.)

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

184841489

Reads aligned (%)

44.9

Duplicates removed (%)

92.5

Number of peaks

1542 (qval < 1E-05)

mm9

Number of total reads

184841489

Reads aligned (%)

45.1

Duplicates removed (%)

90.5

Number of peaks

1760 (qval < 1E-05)