Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR2F2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS derived cardiac cells
NA
NA

Attributes by original data submitter

Sample

source_name
fibroblasts
cell type
hiPSCs-CMs
Stage
day 30
replicate
1
chip-seq antibody
Active Motif, 61213

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-seq: mRNA was extracted using the miRNEASY kit from Qiagen. ChIP-seq: Cells were crosslinked with formalin. Bulk RNA-seq: Ovation® RNA-Seq System V2 kit (NuGEN) and NEBNext DNA Library Prep Master Mix Set for Illumina. Single-cell RNA-seq: SMARTer Ultra Low RNA Kit for the Fluidigm C1 Nextera XT DNA Sample Preparation Kit. Gene edited lines RNA-seq: Ion AmpliSeq™ Transcriptome Human Gene Expression Kit, the Ion PI™ Hi-Q™ Sequencing 200 Kit. ChIP-seq: NEBNext DNA Library Prep Master Mix Set for Illumina or NEBNext® Ultra™ DNA Library Prep.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
36901795
Reads aligned (%)
164.3
Duplicates removed (%)
16.2
Number of peaks
566 (qval < 1E-05)

hg38

Number of total reads
36901795
Reads aligned (%)
166.4
Duplicates removed (%)
15.8
Number of peaks
1270 (qval < 1E-05)

Base call quality data from DBCLS SRA