Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Digestive tract
Cell type
Colon cancer
NA
NA

Attributes by original data submitter

Sample

source_name
T5088-CCICs
ID
T5088
cell type
colon cancer stem cells
sorting marker
CD133

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Millipore EZ-ChIP #17-371 kit (1)DNA-end repair,3'-dA overhang and ligation of methylated sequencing adaptor.(2)PCR amplification and size selection (usually 100-300bp,including adaptorsequence).(3)Qualified library forsequencing. Which follow illumina instruction

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
90260080
Reads aligned (%)
96.4
Duplicates removed (%)
24.7
Number of peaks
1632 (qval < 1E-05)

hg19

Number of total reads
90260080
Reads aligned (%)
95.8
Duplicates removed (%)
25.7
Number of peaks
1649 (qval < 1E-05)

Base call quality data from DBCLS SRA