Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K79me2

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MLL-AF4 rearranged acute myeloid leukemia
cell line
MV4;11(Cas9; ENL-FKBP(F36V); ENL-/-)
input sample
mv411_input
treatment
0.1% DMSO, 500 nM dTAG-13 for 96 hr
growth protocol
RPMI, 10% FBS
sequencing barcode
TCAGGAGG
antibody
H3K79me2
antibody manufacturer
abcam
antibody catalog number
ab3594

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
79622388
Reads aligned (%)
98.1
Duplicates removed (%)
16.1
Number of peaks
10542 (qval < 1E-05)

hg19

Number of total reads
79622388
Reads aligned (%)
97.8
Duplicates removed (%)
16.8
Number of peaks
10476 (qval < 1E-05)

Base call quality data from DBCLS SRA