GSM2183709: mv411 dTAG 96h H3K79me2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K79me2
Cell type
Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
MLL-AF4 rearranged acute myeloid leukemia
cell line
MV4;11(Cas9; ENL-FKBP(F36V); ENL-/-)
input sample
mv411_input
treatment
0.1% DMSO, 500 nM dTAG-13 for 96 hr
growth protocol
RPMI, 10% FBS
sequencing barcode
TCAGGAGG
antibody
H3K79me2
antibody manufacturer
abcam
antibody catalog number
ab3594
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.