Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MLLT1

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MLL-AF4 rearranged acute myeloid leukemia
cell line
MV4;11
input sample
mv411_input
treatment
none
growth protocol
RPMI, 10% FBS
sequencing barcode
TAAGCTTT
antibody
ENL
antibody manufacturer
Cell Signalling
antibody catalog number
14893

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
43013532
Reads aligned (%)
67.6
Duplicates removed (%)
30.8
Number of peaks
2606 (qval < 1E-05)

hg38

Number of total reads
43013532
Reads aligned (%)
69.6
Duplicates removed (%)
29.1
Number of peaks
2158 (qval < 1E-05)

Base call quality data from DBCLS SRA