Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

S2

cell type

embryo-derived

passages

Low passages (6-10)

cell line

S2

chip antibody

H3K27ac (homemade)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA after ChIP protocol was isolated using QIAGEN PCR purification kit. Ribo-Depletion. Input RNA quality was validated using the Agilent RNA 6000 Nano Kit. 200ng-1 µg

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

10337172

Reads aligned (%)

94.1

Duplicates removed (%)

13.2

Number of peaks

10285 (qval < 1E-05)

dm3

Number of total reads

10337172

Reads aligned (%)

94.2

Duplicates removed (%)

12.4

Number of peaks

10541 (qval < 1E-05)