Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 breast cancer cell line
cell line
MCF7
chip antibody
anti-H3K4me1 (05-1248-S) Millipore
cell type
asynchonous cells
treatment
MLL3 siRNA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq, Schmidt et al, 2009. PMID: 19275951 After amplification, DNA was extracted using QIAgen MinElute gel extraction kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
37172124
Reads aligned (%)
97.5
Duplicates removed (%)
3.6
Number of peaks
44163 (qval < 1E-05)

hg19

Number of total reads
37172124
Reads aligned (%)
97.2
Duplicates removed (%)
3.8
Number of peaks
44031 (qval < 1E-05)

Base call quality data from DBCLS SRA