Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GRHL2

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 breast cancer cell line
cell line
MCF7
chip antibody
anti-GRHL2 (HPA004820) Sigma Aldrich
cell type
asynchonous cells
treatment
No treatment

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq, Schmidt et al, 2009. PMID: 19275945 After amplification, DNA was extracted using QIAgen MinElute gel extraction kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
21125238
Reads aligned (%)
97.8
Duplicates removed (%)
19.8
Number of peaks
36100 (qval < 1E-05)

hg19

Number of total reads
21125238
Reads aligned (%)
97.2
Duplicates removed (%)
20.8
Number of peaks
36093 (qval < 1E-05)

Base call quality data from DBCLS SRA