Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GRHL2

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 breast cancer cell line
cell line
MCF7
chip antibody
anti-GRHL2 (HPA004820) Sigma Aldrich
cell type
asynchonous cells
treatment
No treatment

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq, Schmidt et al, 2009. PMID: 19275944 After amplification, DNA was extracted using QIAgen MinElute gel extraction kit.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
53312702
Reads aligned (%)
89.8
Duplicates removed (%)
7.9
Number of peaks
32905 (qval < 1E-05)

hg38

Number of total reads
53312702
Reads aligned (%)
91.5
Duplicates removed (%)
6.6
Number of peaks
32363 (qval < 1E-05)

Base call quality data from DBCLS SRA