Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KDM2B

Cell type

Cell type Class
Blood
Cell type
OCI-LY1
NA
NA

Attributes by original data submitter

Sample

source_name
OCI-Ly1_KDM2B
cell line
OCI-Ly1
cell type
diffuse large B cell lymphoma (DLBCL) cell line
chip antibody
KDM2B

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was purified using QIAGEN MiniElute column after reverse cross-linking. Rabbit polyclonal antibody was raised against human KDM2B and affinity purified (Gearhart, et al. Mol Cell Biol 2006; 26:6880-6889) Libraries were prepared using the Illumina ChIP-Seq sample kits, according to the manufacturer. Libraries were validated using the Agilent Technologies 2100 Bioanalyzer and Quant-iT™ dsDNA HS Assay (Life Technologies)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
184313588
Reads aligned (%)
77.2
Duplicates removed (%)
21.3
Number of peaks
34688 (qval < 1E-05)

hg38

Number of total reads
184313588
Reads aligned (%)
80.0
Duplicates removed (%)
19.1
Number of peaks
35258 (qval < 1E-05)

Base call quality data from DBCLS SRA