Antigen

Antigen Class

TFs and others

Antigen

Rad21

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

Peritoneal macropahge

cell line

Peritoneal macrophage

chip antibody

Rad21, abcam ab992

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, cells were fixed with 2mM disuccinimidyl glutarate (DSG) for 45 mins and 1% formaldehyde for 10mins, and followed by glycine for 5 mins. Chromatin DNA was sheared to 200–500 bp average in size by sonication and chromatin was immunoprecipitated with antibodies. Protein G dynabeads (Invitrogen) were added and incubated overnight at 4°C. After washing and elution, the protein–DNA complex was reverse-crosslinked by heating at 65°C, and immunoprecipitated DNA was purified by using QIAquick Spin column. the libraries were constructed following Illumina's Chip-Seq Sample prep kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

74075503

Reads aligned (%)

83.4

Duplicates removed (%)

56.4

Number of peaks

77057 (qval < 1E-05)

mm9

Number of total reads

74075503

Reads aligned (%)

83.3

Duplicates removed (%)

56.4

Number of peaks

77054 (qval < 1E-05)