Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Breast
Cell type
BT-474
Primary Tissue
Breast
Site of Extraction
Soft Tissue
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
BT-474 cells
treatment
starved, 100uM EGF 90min
factor chip
ERRalpha
tissue of origin
breast
cell line
BT-474

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were extracted from sonicated 1% formaldehyde-crosslinked nuclei and histone-DNA complexes were isolated with ERRalpha antibody. DNA was purified using QIAquick PCR Purification Kit. Libraries were prepared using the Illumina TruSeq ChIP Library Prep Kit Libraries were prepared at McGill University and Génome Québec Innovation Centre according to the Illumina TruSeq ChIP Library Prep Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
65134378
Reads aligned (%)
97.6
Duplicates removed (%)
12.9
Number of peaks
10675 (qval < 1E-05)

hg19

Number of total reads
65134378
Reads aligned (%)
96.6
Duplicates removed (%)
14.6
Number of peaks
11213 (qval < 1E-05)

Base call quality data from DBCLS SRA