GSM2160180: SKBr3 Hrg Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
SK-BR-3
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
SKBr3 cells
treatment
starved, 100uM HRG 90min
factor chip
input
tissue of origin
breast
cell line
SKBr3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were extracted from sonicated 1% formaldehyde-crosslinked nuclei and histone-DNA complexes were isolated with ERRalpha antibody. DNA was purified using QIAquick PCR Purification Kit. Libraries were prepared using the Illumina TruSeq ChIP Library Prep Kit Libraries were prepared at McGill University and Génome Québec Innovation Centre according to the Illumina TruSeq ChIP Library Prep Kit